Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003

Journal of Advanced Research Design
Volume 62, No. 1, November 2019, Pages 10-15

Mohanapriya Hari1, Maegala Nallapan Maniyam1,2,*

1 Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia
2 Center for Foundation and General studies, Universiti Selangor, Jalan Timur Tambahan, 45600 Bestari Jaya, Selangor, Malaysia
*Corresponding author: maegala@unisel.edu.my

KEYWORDS

Decolourization; immobilization; Methylene blue; resting cells; Rhodococcus strain UCC 0003 strain; textile Industry

ABSTRACT

Methylene blue is extensively used in printing and batik industries in Malaysia. The increase in usage of coloured dyes which are toxic and long lasting in the natural environment can affect the water quality. Thus, there is a worthy technique which was developed as a cheaper way to achieve desired environment without hazards. The current study investigated the use of locally isolated Rhodococcus strain UCC 0003 for methylene blue dye decolourization. The decolourization of methylene blue dye was carried out in the two different modes namely resting cells and immobilized cells of Rhodococcus strain UCC 0003 in gellan gum matrix. The methylene blue removal using resting cells and immobilized cells after 24 hours of incubation resulted in 38 % and 73 % decolourization, respectively. Further characterization was proceeded with immobilized cells in gellan gum matrix to evaluate the potential of repeated use of the biocatalyst. The reusability of immobilized cells of Rhodococcus strain UCC 003 was carried out for 17 repeated cycles. The first cycle was initiated by adding 50 mL (0.5 g/L) of methylene blue solution in the Erlemeyer flask that contained 50 beads of gellan gum. The first 16 cycles resulted in complete decolourization within an hour incubation period for each cycle. Meanwhile at the 17th cycle, the decolourization efficiency dropped to 91%. This trend was repeated when fresh cells of Rhodococcus strain UCC 0003 immobilized in gellan gum were tested to decolourize 0.5 g/L of methylene blue. These findings clearly proposed that immobilized cells of Rhodococcus strain UCC 0003 in gellan gum matrix has huge prospects to remediate the industrial wastewater since the biocatalyst could be reused which optimized the cost and method is environmentally acceptable.

CITE THIS ARTICLE

MLA
Mohanapriya, Hari, et al. “Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003.” Journal of Advanced Research Design 62.1 (2019): 10-15.

APA
Mohanapriya, H., & Maegala, N. M.(2019). Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003. Journal of Advanced Research Design, 62(1), 10-15.

Chicago
Mohanapriya Hari, and Maegala Nallapan Maniyam.”Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003.” Journal of Advanced Research Design. 62, no. 1 (2019): 10-15.

Harvard
Mohanapriya, H., and Maegala, N.M., 2019. Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003. Journal of Advanced Research Design 62(1), pp. 10-15.

Vancouver
Mohanapriya H, Maegala NM. Decolourization of Methylene Blue by Resting Cells and Immobilized Cells of Rhodococcus Strain UCC 0003. Journal of Advanced Research Design. 2019;62(1): 10-15.

REFERENCES

[1] Bayat, Zeynab, Mehdi Hassanshahian, and Simone Cappello. “Immobilization of microbes for bioremediation of crude oil polluted environments: a mini review.” The open microbiology journal 9 (2015): 48.
[2] Bhatia, Deepika, Neeta Raj Sharma, Joginder Singh, and Rameshwar S. Kanwar. “Biological methods for textile dye removal from wastewater: A review.” Critical Reviews in Environmental Science and Technology 47, no. 19 (2017): 1836-1876.
[3] Chakraborty, Bhaswati, Lalitagauri Ray, and Srabanti Basu. “Biochemical degradation of Methylene Blue using a continuous reactor packed with solid waste by E. coli and Bacillus subtilis isolated from wetland soil.” Desalination and Water Treatment 57, no. 30 (2016): 14077-14082.
[4] Foster, Alexander, Nicole Barnes, Robert Speight, and Mark A. Keane. “The repertoire of nitrogen assimilation in Rhodococcus: catalysis, pathways and relevance in biotechnology and bioremediation.” Journal of Chemical Technology & Biotechnology 89, no. 6 (2014): 787-802.
[5] Gonçalves, A. I. C., L. A. Rocha, J. M. L. Dias, L. A. Passarinha, and A. Sousa. “Optimization of a chromatographic stationary phase based on gellan gum using central composite design.” Journal of Chromatography B 957 (2014): 46-52.
[6] Górecka, El?bieta, and Magdalena Jastrz?bska. “Immobilization techniques and biopolymer carriers.” Biotechnology and Food Science 75, no. 1 (2011): 65-86.
[7] Holkar, Chandrakant R., Ananda J. Jadhav, Dipak V. Pinjari, Naresh M. Mahamuni, and Aniruddha B. Pandit. “A critical review on textile wastewater treatments: possible approaches.” Journal of environmental management 182 (2016): 351-366.
[8] Rana Rahman, M., and M. K. Neethu. “DEGRADATION OF METHYLENE BLUE IN TEXTILE WASTE WATER USING ACTIVATED SAWDUST AND EGGSHELL BIOSORBENT.”
[9] Maniyam, Maegala Nallapan, Abdul Latif Ibrahim, and Anthony EG Cass. “Enhanced cyanide biodegradation by immobilized crude extract of Rhodococcus UKMP-5M.” Environmental technology 40, no. 3 (2019): 386-398.
[10] Maniyam, Maegala Nallapan, Fridelina Sjahrir, and Mohanapriya Hari. “Decolourization of Methylene Blue by Rhodococcus Strain UCC 0003.” International Journal of Environmental Science and Development 9, no. 11 (2018).
[11] Mohamad, Nur Royhaila, Nur Haziqah Che Marzuki, Nor Aziah Buang, Fahrul Huyop, and Roswanira Abdul Wahab. “An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes.” Biotechnology & Biotechnological Equipment 29, no. 2 (2015): 205-220.
[12] Mohamad, Nur Royhaila, Nur Haziqah Che Marzuki, Nor Aziah Buang, Fahrul Huyop, and Roswanira Abdul Wahab. “An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes.” Biotechnology & Biotechnological Equipment 29, no. 2 (2015): 205-220.
[13] Nath, Jayashree, Arundhati Das, and Lalitagauri Ray. “Biosorption of Malachite Green from Aqueous Solution Using Resting and Immobilised Biomass of Bacillus cereus M116 (MTCC 5521).” Indian Chemical Engineer 57, no. 1 (2015): 82-100.
[14] Sultan, Misbah. “Polyurethane for removal of organic dyes from textile wastewater.” Environmental Chemistry Letters 15, no. 2 (2017): 347-366.
[15] Yu, Lei, Xiao-yu Zhang, Qing-wen Tang, Jia Li, Tian Xie, Chang Liu, Ming-yue Cao et al. “Decolorization characteristics of a newly isolated salt-tolerant Bacillus sp. strain and its application for azo dye-containing wastewater in immobilized form.” Applied microbiology and biotechnology 99, no. 21 (2015): 9277-9287.