Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate

Journal of Advanced Research Design
Volume 62, No. 1, November 2019, Pages 1-9

Anupriya Sundarajoo1, Maegala Nallapan Maniyam1,2,*

1 Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia
2 Center for Foundation and General studies, Universiti Selangor, Jalan Timur Tambahan, 45600 Bestari Jaya, Selangor, Malaysia
*Corresponding author: maegala@unisel.edu.my


Congo red, Rhodococcus, dye decolourization, immobilization


The world’s once glorious environment is heavily impacted by pollution caused by many industrial activities. The textile industry in particular has its share in pollution of water, land and air. With limited water resources and fast emerging nature of the textile industry in our nation, this raises concern in regards to detrimental effect of improper textile industry waste management. This waste is toxic and noxious which negatively affect living organisms and the environment when discharged without proper treatment. Utilization of microorganism through green chemistry approach is an alternative for industrial application to be sustainable, eco-friendly, cost- and time-effective compared to conventional effluent treatment methods that have considerable drawbacks. This study is interested in subjecting Rhodococcus sp. that is sturdy and resilient to decolourize di-azo dye Congo red, a highly recalcitrant and toxic dye since no previous studies have been reported with similar objectives. Screening studies showed 8 strains were capable of forming halo zone around colony on 0.1 g/L Congo red agar plate with UCC 0010 giving largest halo zone with 1 cm diameter at day 5 of incubation. Similar results were obtained for Congo red decolourization in 24 hours at static condition for resting cells and heat-killed cells with 46d ± 1.5 % and 46d ± 7 % dye removal respectively. This shows dye removal is majorly via biosorption since cells were deeply coloured. Live cells yielded higher decolourization compared to resting cells with 67c ± 1.7% decolourization, possibly due to active metabolic state. Cells immobilized in both calcium alginate and gellan gum showed significantly higher decolourization and biosorption to matrix in control set. However calcium alginate is selected as best entrapment matrix since drastic difference was seen between control set and dye solution inoculated with biobeads (beads containing UCC 0010) indicating enzymatic action for Congo red decolourization.


Anupriya, Sundarajoo, et al. “Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate.” Journal of Advanced Research Design 62.1 (2019): 1-9.

Anupriya, S., & Maegala, N. M.(2019). Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate. Journal of Advanced Research Design, 62(1), 1-9.

Anupriya Sundarajoo, and Maegala Nallapan Maniyam.”Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate.” Journal of Advanced Research Design. 62, no. 1 (2019): 1-9.

Anupriya, S., and Maegala, N.M., 2019. Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate. Journal of Advanced Research Design 62(1), pp. 1-9.

Anupriya S, Maegala NM. Enhanced Decolourization of Congo Red Dye by Malaysian Rhodococcus UCC 0010 Immobilized in Calcium Alginate. Journal of Advanced Research Design. 2019;62(1): 1-9.


[1] Allam, Nanis. “Bioremediation Efficiency of Heavy Metals and Azo Dyes by Individual or Consortium Bacterial Species Either as Free orImmobilized Cells: A Comparative Study.” Egyptian Journal of Botany 57, no. 3 (2017): 555-564.
[2] Ani. (2018). Hundreds of fish found dead in Coimbatore’s Valankulam tank. Retrieved from https://in.news.yahoo.com/hundreds-fish-found-dead-coimbatores-020200953.html
[3] Chakraborty, Samayita, Bikram Basak, Subhasish Dutta, Biswanath Bhunia, and Apurba Dey. “Decolorization and biodegradation of congo red dye by a novel white rot fungus Alternaria alternata CMERI F6.” Bioresource technology 147 (2013): 662-666.
[4] De Carvalho, Carla CCR, Sofia Santos Costa, Pedro Fernandes, Isabel Couto, and Miguel Viveiros. “Membrane transport systems and the biodegradation potential and pathogenicity of genus Rhodococcus.” Frontiers in physiology 5 (2014): 133.
[5] E?, Ismail, José Daniel Gonçalves Vieira, and André Corrêa Amaral. “Principles, techniques, and applications of biocatalyst immobilization for industrial application.” Applied microbiology and biotechnology 99, no. 5 (2015): 2065-2082.
[6] Guardian staff. (2017). The blue dogs of Mumbai: industrial waste blamed for colourful canines. Retrieved from https://www.theguardian.com/world/2017/aug/22/the-blue-dogs-of-mumbai-industrial-waste-blamed-for-colourful-canines
[7] Hassaan, Mohamed A., and Ahmed El Nemr. “Health and environmental impacts of dyes: mini review.” American Journal of Environmental Science and Engineering 1, no. 3 (2017): 64-67.
[8] Holkar, Chandrakant R., Ananda J. Jadhav, Dipak V. Pinjari, Naresh M. Mahamuni, and Aniruddha B. Pandit. “A critical review on textile wastewater treatments: possible approaches.” Journal of environmental management 182 (2016): 351-366.
[9] Lade, Harshad, Sanjay Govindwar, and Diby Paul. “Mineralization and detoxification of the carcinogenic azo dye Congo red and real textile effluent by a polyurethane foam immobilized microbial consortium in an upflow column bioreactor.” International journal of environmental research and public health 12, no. 6 (2015): 6894-6918.
[10] Maniyam, Maegala Nallapan, Abdul Latif Ibrahim, and Anthony EG Cass. “Decolourization and biodegradation of azo dye methyl red by Rhodococcus strain UCC 0016.” Environmental technology 41, no. 1 (2020): 71-85.
[11] Maniyam, Maegala Nallapan, Fridelina Sjahrir, and Mohanapriya Hari. “Decolourization of Methylene Blue by Rhodococcus Strain UCC 0003.” International Journal of Environmental Science and Development 9, no. 11 (2018).
[12] Maniyam, Maegala Nallapan, Nor Suhaila Yaacob, Hazeeq Hazwan Azman, Noor Azmahera Ab Ghaffar, and Hasdianty Abdullah. “Immobilized cells of Rhodococcus strain UCC 0004 as source of green biocatalyst for decolourization and biodegradation of methyl orange.” Biocatalysis and agricultural biotechnology 16 (2018): 569-578.
[13] Ng, I-Son, Tingting Chen, Rong Lin, Xia Zhang, Chao Ni, and Dongzhe Sun. “Decolorization of textile azo dye and Congo red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BC01.” Applied microbiology and biotechnology 98, no. 5 (2014): 2297-2308.
[14] Pang, Yean L., and Ahmad Z. Abdullah. “Current status of textile industry wastewater management and research progress in Malaysia: a review.” Clean–Soil, Air, Water 41, no. 8 (2013): 751-764.
[15] Park, Eun-Hee, Moon-Sun Jang, In-Ho Cha, YONG-LARK Choi, Young-Su Cho, Cheorl Ho Kim, and YOUNG-CHOON Lee. “Decolorization of a sulfonated azo dye, Congo Red, by Staphylococcus sp. EY-3.” Journal of microbiology and biotechnology 15, no. 1 (2005): 221-225.
[16] Roy, Uttariya, Suvendu Manna, Shubhalakshmi Sengupta, Papita Das, Siddhartha Datta, Aniruddha Mukhopadhyay, and Avijit Bhowal. “Dye removal using microbial biosorbents.” In Green Adsorbents for Pollutant Removal, pp. 253-280. Springer, Cham, 2018.
[17] Sarim, Khan Mohd, Kamlesh Kukreja, Ikbal Shah, and Chetan K. Choudhary. “Biosorption of direct textile dye Congo red by Bacillus subtilis HAU-KK01.” Bioremediation Journal 23, no. 3 (2019): 185-195.
[18] Shahid, S. N. (2018). Dark Flows the River Turag: Witnessing the Environmental Perils of Bangladesh’s Textile Industry. Retrieved from https://www.thedailystar.net/star-weekend/environment/dark-flows-the-river-turag-1587946
[19] Tokiran, Shasila, Maegala Nallapan Maniyam, Nor Suhaila Yaacob, and Abdul Latif Ibrahim. “Decolourization of textile dyes by Malaysian Rhodococcus strains.” Indian Journal of Fundamental and Applied Life Sciences 6 (2016): 14-20.
[20] Singh, Rajat Pratap, Pradeep Kumar Singh, Rasna Gupta, and Ram Lakhan Singh. “Treatment and recycling of wastewater from textile industry.” In Advances in Biological Treatment of Industrial Waste Water and their Recycling for a Sustainable Future, pp. 225-266. Springer, Singapore, 2019.
[21] Malaysian Investment Development Authority (2018). Textiles and textile products. Retrieved from http://www.mida.gov.my/home/textiles-and-textile-products/posts/