Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool

Journal of Advanced Research Design
Volume 63, No. 1, November 2019, Pages 11-17

Kanchana Rajendran1, Maegala NallapanManiya2,*

1 Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia
2 Centre for Foundation and General Studies, Universiti Selangor, Jalan Timur Tambahan, 45600 Bestari Jaya, Selangor, Malaysia
*Corresponding author: maegala@unisel.edu.my

KEYWORDS

biodegradation; crystal violet; decolourization, immobilization, Rhodococcus; textile industry; water pollution

ABSTRACT

Every year million tons of dyes and toxic compounds are discharged as industrial waste which pose a threat to the environment and human health. Thus, environmental concern over discharge of coloured wastewater has been on the rise. A competent method which is environmentally acceptable and economically viable is desired to treat the textile effluent prior to discharge into the environment. In the present study, twenty-three locally isolated Rhodococcus strains were examined as biological tools for decolourization of crystal violet. The tested microorganisms which were locally isolated Rhodococcus strains demonstrated promising ability to decolourize crystal violet as evidenced by the change in the colour of the dye from dark purple to pale purple on nutrient agar plates. One of the best strains namely Rhodococcus strain UCC 0004 formed clear zone around the colonies of the bacteria after 5 days of incubation period. This strain demonstrated good growth and completely decolourized 0.6 mM crystal violets after 5 days of incubation period. Further investigation was carried out by comparing the ability between immobilized cells and whole cells of Rhodococcus strain UCC 0004 for efficient crystal violet removal. The results showed that the percentage of crystal violet decolourization was highest ability to degraded when immobilized cells in gellan gum (99 a ± 0 %) and calcium alginate (98a ± 2 %), were used compared to heat killed cells which yielded (11c ± 0 %) of crystal violet removal. These findings clearly indicated that the immobilized cell of Rhodococcus strain UCC 0004 has a huge potential as biological tool to remediate actual wastewater containing crystal violet.

CITE THIS ARTICLE

MLA
Kanchana, Rajendran, et al. “Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool.” Journal of Advanced Research Design 63.1 (2019): 11-17.

APA
Kanchana, R., & Maegala, N.(2019). Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool. Journal of Advanced Research Design, 63(1), 11-17.

Chicago
Kanchana Rajendran, and Maegala NallapanManiya.”Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool.” Journal of Advanced Research Design. 63, no. 1 (2019): 11-17.

Harvard
Kanchana, R., and Maegala, N., 2019. Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool. Journal of Advanced Research Design 63(1), pp. 11-17.

Vancouver
Kanchana R, Maegala N. Enhanced Biodegradation of Crystal Violet by Immobilized Cells in Rhodococcus Strain ucc 0004 as Biological Tool. Journal of Advanced Research Design. 2019;63(1): 11-17.

REFERENCES

[1] Azmi, Wamik, Rajesh Kumar Sani, and Uttam Chand Banerjee. “Biodegradation of triphenylmethane dyes.” Enzyme and microbial technology 22, no. 3 (1998): 185-191.
[2] Bharagava, Ram Naresh, Sujata Mani, Sikandar I. Mulla, and Ganesh Dattatraya Saratale. “Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation.” Ecotoxicology and environmental safety 156 (2018): 166-175.
[3] Chiing-Chang C, Hung-Ju L, Chiu-Yu C, Chia-Yuen Y, Ying-Chien C (2015). Degradation and decolourization potential of an ligninolytic enzyme producing Aeromonas hydrophila for crystal violet dye and its phytotoxicity evaluation.
[4] De Carvalho, Carla CCR, Sofia Santos Costa, Pedro Fernandes, Isabel Couto, and Miguel Viveiros. “Membrane transport systems and the biodegradation potential and pathogenicity of genus Rhodococcus.” Frontiers in physiology 5 (2014): 133.
[5] Galai, Said, Ferid Limam, and M. Nejib Marzouki. “Decolorization of an industrial effluent by free and immobilized cells of Stenotrophomonas maltophilia AAP56. Implementation of efficient down flow column reactor.” World Journal of Microbiology and Biotechnology 26, no. 8 (2010): 1341-1347.
[6] Gonçalves, A. I. C., L. A. Rocha, J. M. L. Dias, L. A. Passarinha, and A. Sousa. “Optimization of a chromatographic stationary phase based on gellan gum using central composite design.” Journal of Chromatography B 957 (2014): 46-52.
[7] Tan, Liang, Hua Li, Shuxiang Ning, and Bingwen Xu. “Aerobic decolorization and degradation of azo dyes by suspended growing cells and immobilized cells of a newly isolated yeast Magnusiomyces ingens LH-F1.” Bioresource technology 158 (2014): 321-328.
[8] Maniyam, Maegala Nallapan, Abdul Latif Ibrahim, and Anthony EG Cass. “Decolourization and biodegradation of azo dye methyl red by Rhodococcus strain UCC 0016.” Environmental technology 41, no. 1 (2020): 71-85.
[9] Maniyam, Maegala Nallapan, Fridelina Sjahrir, and Mohanapriya Hari. “Decolourization of Methylene Blue by Rhodococcus Strain UCC 0003.” International Journal of Environmental Science and Development 9, no. 11 (2018).
[10] Mani, Sujata, and Ram Naresh Bharagava. “Exposure to crystal violet, its toxic, genotoxic and carcinogenic effects on environment and its degradation and detoxification for environmental safety.” In Reviews of Environmental Contamination and Toxicology Volume 237, pp. 71-104. Springer, Cham, 2016.
[11] Parshetti, G. K., S. G. Parshetti, A. A. Telke, D. C. Kalyani, R. A. Doong, and Sanjay Prabhu Govindwar. “Biodegradation of crystal violet by Agrobacterium radiobacter.” Journal of environmental sciences 23, no. 8 (2011): 1384-1393.
[12] Pearce, C. I., J. R. Lloyd, and J. T. Guthrie. “The removal of colour from textile wastewater using whole bacterial cells: a review.” Dyes and pigments 58, no. 3 (2003): 179-196.
[13] Tokiran, Shasila, Maegala Nallapan Maniyam, Nor Suhaila Yaacob, and Abdul Latif Ibrahim. “Decolourization of textile dyes by Malaysian Rhodococcus strains.” Indian Journal of Fundamental and Applied Life Sciences 6 (2016): 14-20.
[14] Shah, Maulin P., Kavita A. Patel, Sunu S. Nair, and Darji AM. “Microbiological removal of crystal violet dye by Bacillus subtilis ETL-2211.” OA Biotechnol 2, no. 9 (2013).
[15] Telke, Amar A., Dayanand C. Kalyani, Vishal V. Dawkar, and Sanjay P. Govindwar. “Influence of organic and inorganic compounds on oxidoreductive decolorization of sulfonated azo dye CI Reactive Orange 16.” Journal of hazardous materials 172, no. 1 (2009): 298-309.
[16] Pang, Yean L., and Ahmad Z. Abdullah. “Current status of textile industry wastewater management and research progress in Malaysia: a review.” Clean–Soil, Air, Water 41, no. 8 (2013): 751-764.