Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products
Journal of Advanced Research in Fluid Mechanics and Thermal Sciences
Volume 42 No. 1, February 2018, Pages 72-79
Rabieatul Adawieah Md Akhir1, Mohd Fadzelly Abu Bakar1,2,*, Shuaibu Babaji Sanusi1,2
1Faculty of Science, Technology & Human Development, Universiti Tun Hussein Onn Malaysia (UTHM), 86400 Parit Raja, Batu Pahat, Johor, Malaysia
2Centre of Research- Sustainable Research of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), 86400 Parit Raja, Batu Pahat, Johor, Malaysia
*Corresponding author: email@example.com
Stingless bee, bee bread, propolis, antioxidant activity, antimicrobial activity
Bee bread is the pollen that has been stored in the cells of the honeycomb with various enzymes and honey, which undergoes lactic acid fermentation, while propolis is a resinous material collected by bees from buds of the plants and transform it using bee enzymes. The main objective of this research was to investigate the antioxidant and antimicrobial activity of the stingless bee bread and propolis extracted using 70% ethanol and n-hexane. The antioxidant activity of sample extracts were determined by 2,2,-di-phenyl-2-picryl-hydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and Ferric Reducing Antioxidant Power (FRAP) assays while for the antimicrobial activity, the sample extracts were analyzed using disc diffusion and broth macrodilution assay. For the DPPH and ABTS assays, the results showed that ethanolic extract of bee bread showed the highest percentage of free radical scavenging as compared to other samples with the values of 93.60 ± 0.03% and 97.95±0.01%, respectively. However, FRAP value for both hexanic extract samples, bee bread and propolis have the values of 0.85±0.0 mM FE/g and 0.87±0.00 mM FE/g respectively which were higher than ethanolic extract samples, 2.41±0.02 mM FE/g (bee bread) and 6.64±0.04 mM FE/g (propolis). For disc diffusion assay, the results showed that ethanolic extract of bee bread and propolis as well as hexanic extract of propolis were able to inhibit all tested bacteria in varying diameter of the inhibition zone. Broth macrodilution assay showed minimum inhibition zone (MIC) ranging from <6.67 to 33.33 µL/mL. As the conclusion, all of the samples in this study displayed antioxidant and antimicrobial effect but there are different in the degree of antioxidant and antimicrobial activity exhibited between each of the samples.
CITE THIS ARTICLE
Md Akhir, Rabieatul Adawieah, et al. “Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products.” Journal of Advanced Research in Fluid Mechanics and Thermal Sciences 42.1 (2018): 72-79.
Md Akhir, R. A., Abu Bakar, M. F., & Sanusi, S. B. (2018). Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 42(1), 72-79.
Md Akhir, Rabieatul Adawieah, Mohd Fadzelly Abu Bakar, and Shuaibu Babaji Sanusi. “Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products.” Journal of Advanced Research in Fluid Mechanics and Thermal Sciences 42, no. 1 (2018): 72-79.
Md Akhir, R.A., Abu Bakar, M.F. and Sanusi, S.B., 2018. Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 42(1), pp.72-79.
Md Akhir, RA, Abu Bakar, MF, Sanusi, SB. Antioxidant and Antimicrobial Potential of Stingless Bee (Heterotrigona itama) By-Products. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences. 2018;42(1):72-79.
 Rasmussen, Claus, and Sydney A. Cameron. “Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal.” Biological Journal of the Linnean Society 99, no. 1 (2009): 206-232.
 Kelly, N., M. S. N. Farisya, T. K. Kumara, and P. Marcela. “Tropical Agricultural Science.” Pertanika J. Trop. Agric. Sci 37, no. 3 (2014): 293-298.
 Nagai, Takeshi, Toshio Nagashima, Nobutaka Suzuki, and Reiji Inoue. “Antioxidant activity and angiotensin Iconverting enzyme inhibition by enzymatic hydrolysates from bee bread.” Zeitschrift für Naturforschung C 60, no. 1-2 (2005): 133-138.
 Yap, Philip, Mohd Fadzelly Abu Bakar, Herbert Lim, and David Carrier. “Antibacterial activity of polyphenol-rich extract of selected wild honey collected in Sabah, Malaysia.” Journal of Apicultural Research 54, no. 3 (2015): 163-172.
 Philip, Y., and A. B. Mohd Fadzelly. “Antioxidative and acetylcholinesterase inhibitor potential of selected honey of Sabah, Malaysian Borneo.” International Food Research Journal 22, no. 5 (2015).
 Kustiawan, Paula M., Songchan Puthong, Enos T. Arung, and Chanpen Chanchao. “In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines.” Asian Pacific journal of tropical biomedicine 4, no. 7 (2014): 549-556.
 Missima, Fabiane, and José Maurício Sforcin. “Green Brazilian propolis action on macrophages and lymphoid organs of chronically stressed mice.” Evidence-Based Complementary and Alternative Medicine 5, no. 1 (2008): 71-75.
 GONÇALVES, GISELE, N. P. Santos, and S. M. Srebernich. “Antioxidant and antimicrobial activities of propolis and acai (Euterpe oleracea Mart) extracts.” Revista de Ciências Farmacêuticas Básica e Aplicada 32, no. 3 (2012): 349-356.
 Kurek-Górecka, Anna, Anna Rzepecka-Stojko, Micha? Górecki, Jerzy Stojko, Marian Sosada, and Gra?yna ?wierczek-Zi?ba. “Structure and antioxidant activity of polyphenols derived from propolis.” Molecules 19, no. 1 (2013): 78-101.
 Campos, Jaqueline Ferreira, Uilson Pereira dos Santos, Luis Fernando Benitez Macorini, Adriana Mary Mestriner Felipe de Melo, José Benedito Perrella Balestieri, Edgar Julian Paredes-Gamero, Claudia Andrea Lima Cardoso, Kely de Picoli Souza, and Edson Lucas dos Santos. “Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae).” Food and Chemical Toxicology 65 (2014): 374-380.
 Ivanišová, Eva, Miroslava Ka?ániová, Helena Fran?áková, Jana Petrová, Jana Hutková, Valeryii Brovarskyi, Serhii Velychko, Leonora Adamchuk, Zuzana Schubertová, and Janette Musilová. “Bee bread-perspective source of bioactive compounds for future.” Potravinarstvo Slovak Journal of Food Sciences 9, no. 1 (2015): 592-598.
 Ristivojevi?, Petar, Ivica Dimki?, Jelena Trifkovi?, Tanja Beri?, Irena Vovk, Dušanka Milojkovi?-Opsenica, and Slaviša Stankovi?. “Antimicrobial activity of Serbian propolis evaluated by means of MIC, HPTLC, bioautography and chemometrics.” PloS one 11, no. 6 (2016): e0157097.
 Eswaran, V. U., and H. R. Bhargava. “Chemical Analysis and Anti-Microbial Activity of Karnataka Bee Bread of Apis species.” World Applied Sciences Journal 32, no. 3 (2014): 379-385.
 Choi, Y. M., D. O. Noh, S. Y. Cho, H. J. Suh, K. M. Kim, and J. M. Kim. “Antioxidant and antimicrobial activities of propolis from several regions of Korea.” LWT-Food Science and Technology 39, no. 7 (2006): 756-761.
 Choudhari, Milind K., Sachin A. Punekar, Ramchandra V. Ranade, and Kishore M. Paknikar. “Antimicrobial activity of stingless bee (Trigona sp.) propolis used in the folk medicine of Western Maharashtra, India.” Journal of ethnopharmacology 141, no. 1 (2012): 363-367.
 Isidorov, V. A., A. G. Isidorova, L. Sczczepaniak, and U. Czy?ewska. “Gas chromatographic–mass spectrometric investigation of the chemical composition of beebread.” Food Chemistry 115, no. 3 (2009): 1056-1063.
 Aralas, Sitti, Maryati Mohamed, and Mohd Fadzelly Abu Bakar. “Antioxidant properties of selected salak (Salacca zalacca) varieties in Sabah, Malaysia.” Nutrition & Food Science 39, no. 3 (2009): 243-250.
 Re, Roberta, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang, and Catherine Rice-Evans. “Antioxidant activity applying an improved ABTS radical cation decolorization assay.” Free radical biology and medicine 26, no. 9-10 (1999): 1231-1237.
 Benzie, Iris FF, and John J. Strain. “The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay.” Analytical biochemistry 239, no. 1 (1996): 70-76.
 Hassanain ATa, Alyaa AKb, and Karim AJc. “Antimicrobial effect of Malaysian honey on some human pathogens: An in vitro study.” (2010).
 Chen, Sharon CA, Maryann L. O’donnell, Suzannah Gordon, and Gwendolyn L. Gilbert. “Antifungal susceptibility testing using the E test: comparison with the broth macrodilution technique.” Journal of Antimicrobial Chemotherapy 37, no. 2 (1996): 265-273.
 Baltrušaityt?, Vilma, Petras Rimantas Venskutonis, and Violeta ?eksteryl?. “Antibacterial Activity of Honey and Beebread of Different Origin Against S. aureus and S. epidermidis.” Food Technology & Biotechnology 45, no. 2 (2007).
 Silva, Tania Maria Sarmento, Celso Amorim Camara, Antonio Claudio da Silva Lins, José Maria Barbosa-Filho, Eva Mônica Sarmento da Silva, Breno Magalhães Freitas, and Francisco de Assis Ribeiro dos Santos. “Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke.” Journal of food composition and analysis 19, no. 6-7 (2006): 507-511.
 Leja, M., A. Mareczek, G. Wy?golik, J. Klepacz-Baniak, and K. Czeko?ska. “Antioxidative properties of bee pollen in selected plant species.” Food chemistry 100, no. 1 (2007): 237-240.
 Brand-Williams, Wendy, Marie-Elisabeth Cuvelier, and C. L. W. T. Berset. “Use of a free radical method to evaluate antioxidant activity.” LWT-Food science and Technology 28, no. 1 (1995): 25-30.
 Hamburger, Matthias, and Kurt Hostettmann. “7. Bioactivity in plants: the link between phytochemistry and medicine.” Phytochemistry 30, no. 12 (1991): 3864-3874.
 Machado, Bruna Aparecida Souza, Rejane Pina Dantas Silva, Gabriele de Abreu Barreto, Samantha Serra Costa, Danielle Figueredo da Silva, Hugo Neves Brandão, José Luiz Carneiro da Rocha et al. “Chemical composition and biological activity of extracts obtained by supercritical extraction and ethanolic extraction of brown, green and red propolis derived from different geographic regions in Brazil.” PLoS One 11, no. 1 (2016): e0145954.
 Alves de Souza, Silvana, Celso Amorim Camara, Eva Monica Sarmento da Silva, and Tania Maria Sarmento Silva. “Composition and antioxidant activity of geopropolis collected by Melipona subnitida (Jandaíra) bees.” Evidence Based Complementary and Alternative Medicine 2013 (2013).
 Arnao, Marino B. “Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case.” Trends in Food Science & Technology 11, no. 11 (2000): 419-421.
 Prior, Ronald L., Xianli Wu, and Karen Schaich. “Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements.” Journal of agricultural and food chemistry 53, no. 10 (2005): 4290-4302.
 M?rghita?, Liviu A., Oltica G. Stanciu, Daniel S. Dezmirean, Otilia Bobi?, Olimpia Popescu, Stefan Bogdanov, and Maria Graca Campos. “In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania.” Food Chemistry 115, no. 3 (2009): 878-883.
 Jug, Mario, Marijana Zovko Kon?i?, and Ivan Kosalec. “Modulation of antioxidant, chelating and antimicrobial activity of poplar chemo-type propolis by extraction procures.” LWT-Food Science and Technology 57, no. 2 (2014): 530-537.
 Mohapatra, D. P., V. Thakur, and S. K. Brar. “Antibacterial efficacy of raw and processed honey.” Biotechnology research international 2011 (2011).