Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator
Journal of Advanced Research in Fluid Mechanics and Thermal Sciences
Volume 57, No. 1, May 2019, Pages 1-11
Ummikalsom Abidin1, Burhanuddin Yeop Majlis2,*, Jumril Yunas2
1 Department of Thermofluids, School of Mechanical Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
2 Institute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
*Corresponding author: firstname.lastname@example.org
Ummikalsom, Abidin, et al. "Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator." Journal of Advanced Research in Fluid Mechanics and Thermal Sciences 57.1 (2019): 1-11.
Ummikalsom, A., Burhanuddin, Y. M., & Jumril, Y.(2019). Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 57(1), 1-11.
Ummikalsom Abidin, Burhanuddin Yeop Majlis, and Jumril Yunas."Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator." Journal of Advanced Research in Fluid Mechanics and Thermal Sciences. 57, no. 1 (2019): 1-11.
Ummikalsom, A., Burhanuddin, Y.M., Jumril, Y., 2019. Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences 57(1), pp. 1-11.
Ummikalsom A, Burhanuddin YM, Jumril Y. Efficient Magnetic Microbeads Trapping using Lab-on-Chip Magnetic Separator. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences. 2019;57(1): 1-11.
Lab-on-Chip (LoC); magnetic separator; microfluidics; magnetic microbeads; trapping efficiency
Lab-on-Chip (LoC) magnetic separation is a simple and effective method in separating bioparticles labelled with magnetic microbeads in microfluidics flow condition. In this work, trapping efficiency of magnetic microbeads using LoC magnetic separator and a microfluidics channel with chamber design is determined. The polydimethylsiloxane (PDMS) microfluidics channel was designed with an inlet, an outlet and a circular trapping chamber at the center. Standard soft lithography technique was used to replicate the PDMS microfluidics channel from the SU-8 mould. In a continuous hydrodynamics flow of 1.0 ?L/min, trapping efficiency of 99.5 % and 94.9 % for 4.5 ?m and 2.5 µm magnetic microbeads respectively was achieved. Flow analysis using COMSOL Multiphysics has been conducted in predicting the possible location of the magnetic beads trapping inside the microfluidics channel. The trapping is possible whenever the magnetic force is larger than the drag force experience by the magnetic microbead. The microfluidics channel with chamber design had facilitated low hydrodynamics drag force on the magnetic beads and resulted high efficiency trapping. Therefore, the development of this LoC magnetic separator may be promising to be utilized for biological studies and point-of-care testing (POCT) applications.
 Cavazos, Jessy. 2012. "T&M Supports The Medical Industry’s Transformation." Electronic Design. http://electronicdesign.com/test-amp-measurement/tm-supports-medical-industry-s-transformation.
 Van Reenen, Alexander, Arthur M. de Jong, Jaap MJ den Toonder, and Menno WJ Prins. "Integrated lab-on-chip biosensing systems based on magnetic particle actuation–a comprehensive review." Lab on a Chip 14, no. 12 (2014): 1966-1986.
 Pamme, Nicole. "Magnetism and microfluidics." Lab on a Chip 6, no. 1 (2006): 24-38.
 Cao, Quanliang, Xiaotao Han, and Liang Li. "Configurations and control of magnetic fields for manipulating magnetic particles in microfluidic applications: magnet systems and manipulation mechanisms." Lab on a Chip 14, no. 15 (2014): 2762-2777.
 Guo, S. S., C. C. Zuo, W. H. Huang, C. Peroz, and Y. Chen. "Response of super-paramagnetic beads in microfluidic devices with integrated magnetic micro-columns." Microelectronic engineering 83, no. 4-9 (2006): 1655-1659.
 Han, Ki-Ho, and A. Bruno Frazier. "Paramagnetic capture mode magnetophoretic microseparator for high efficiency blood cell separations." Lab on a Chip 6, no. 2 (2006): 265-273.
 Bu, Minqiang, Troels B. Christensen, Kristian Smistrup, Anders Wolff, and Mikkel F. Hansen. "Characterization of a microfluidic magnetic bead separator for high-throughput applications." Sensors and Actuators A: Physical 145 (2008): 430-436.
 Yu, Xu, Xuan Feng, Jun Hu, Zhi-Ling Zhang, and Dai-Wen Pang. "Controlling the magnetic field distribution on the micrometer scale and generation of magnetic bead patterns for microfluidic applications." Langmuir 27, no. 8 (2011): 5147-5156.
 Teste, Bruno, Florent Malloggi, Anne-Laure Gassner, Thomas Georgelin, Jean-Michel Siaugue, Anne Varenne, Hubert Girault, and Stéphanie Descroix. "Magnetic core shell nanoparticles trapping in a microdevice generating high magnetic gradient." Lab on a Chip 11, no. 5 (2011): 833-840.
 Abidin, Ummikalsom, Burhanuddin Yeop Majlis, and Jumril Yunas. "Integrated high magnetic gradient system for trapping nanoparticles." Jurnal Teknologi 75, no. 11 (2015).
 Abidin, Ummikalsom, Burhanuddin Yeop Majlis, and Jumril Yunas. "Design and simulation of high magnetic gradient device for effective bioparticles trapping." In 2012 10th IEEE International Conference on Semiconductor Electronics (ICSE), pp. 195-199. IEEE, 2012.
 Bruus, H. "Chapter 1 Governing Equations in Microfluidics, 2015." 1-28.
 Abidin, Ummikalsom, Jumril Yunas, and Burhanuddin Yeop Majlis. "Fabrication and testing of polydimethylsiloxane (PDMS) microchannel for lab-on-chip (LOC) magnetically-labelled biological cells separation." Jurnal Teknologi 78, no. 8-4 (2016).
 Spherotech. 2014. "Magnetic Microparticles and Nanoparticles - Spherotech." http://www.spherotech.com/para_par.htm.
 Cadena-Herrera, Daniela, Joshua E. Esparza-De Lara, Nancy D. Ramírez-Ibañez, Carlos A. López-Morales, Néstor O. Pérez, Luis F. Flores-Ortiz, and Emilio Medina-Rivero. "Validation of three viable-cell counting methods: manual, semi-automated, and automated." Biotechnology Reports 7 (2015): 9-16.
 Hafner, E., and P. Chapman. 2011. "Accurate Counting of Bio-Plex® Magnetic or Polystyrene Beads Using the TC10TM Automated Cell Counter | Bioradiations." http://www.bioradiations.com/accurate-counting-of-bio-plex-magnetic-or-polystyrene-beads-using-the-tc10-automated-cell-counter/.
 Ramadan, Qasem, Victor D. Samper, Daniel Poenar Puiu, and Chen Yu. "Fabrication of three-dimensional magnetic microdevices with embedded microcoils for magnetic potential concentration." Journal of microelectromechanical systems15, no. 3 (2006): 624-638.
 Bastidas, Oscar. 2015. "Cell Counting Neubauer Chamber, Celeromics." http://celeromics.com/en/resources/docs/Articles/Cell-counting-Neubauer-chamber.php.
 “Neubauer-Chamber-Cell-Concentration.” 2018. Accessed August 29. http://www.celeromics.com/en/resources/Technical Notes/neubauer-chamber-cell-concentration/neubauer-chamber-cell-concentration.php.
 Hemocytometer. 2015. “Hemocytometer Calculator | Hemocytometer.” http://www.hemocytometer.org/hemocytometer-calculator/.
 Ramadan, Qasem, and Martin AM Gijs. "Simultaneous sample washing and concentration using a “trapping-and-releasing” mechanism of magnetic beads on a microfluidic chip." Analyst136, no. 6 (2011): 1157-1166.
 Fulcrand, Rémy, Aurélien Bancaud, Christophe Escriba, Qihao He, Samuel Charlot, Ali Boukabache, and Anne-Marie Gué. "On chip magnetic actuator for batch-mode dynamic manipulation of magnetic particles in compact lab-on-chip." Sensors and Actuators B: Chemical 160, no. 1 (2011): 1520-1528.
 Ramadan, Qasem, Daniel P. Poenar, and Chen Yu. "Customized trapping of magnetic particles." Microfluidics and nanofluidics 6, no. 1 (2009): 53-62.
 Hejazian, Majid, Weihua Li, and Nam-Trung Nguyen. "Lab on a chip for continuous-flow magnetic cell separation." Lab on a Chip 15, no. 4 (2015): 959-970.
 Wu, Xinyu, Huiying Wu, and Yandong Hu. "Enhancement of separation efficiency on continuous magnetophoresis by utilizing L/T-shaped microchannels." Microfluidics and nanofluidics 11, no. 1 (2011): 11-24.
 Smistrup, Kristian, Ole Hansen, Henrik Bruus, and Mikkel F. Hansen. "Magnetic separation in microfluidic systems using microfabricated electromagnets—experiments and simulations." Journal of Magnetism and Magnetic Materials293, no. 1 (2005): 597-604.
 Lund-Olesen, Torsten, Henrik Bruus, and Mikkel Fougt Hansen. "Quantitative characterization of magnetic separators: comparison of systems with and without integrated microfluidic mixers." Biomedical microdevices 9, no. 2 (2007): 195-205.
 Fulcrand, R., D. Jugieu, C. Escriba, A. Bancaud, D. Bourrier, A. Boukabache, and A. M. Gué. "Development of a flexible microfluidic system integrating magnetic micro-actuators for trapping biological species." Journal of Micromechanics and Microengineering 19, no. 10 (2009): 105019.