Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains

Authors

  • Micky Vincent Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Latifah Suali Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Afizul Safwan Azahari Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Patricia Rowena Mark Baran Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Elexson Nillian Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Lesley Maurice Bilung Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

Keywords:

Lipomyces starkeyi, random mutagenesis, oleaginous yeasts, growth kinetics, fungal biomass

Abstract

Yeast growth and biomass production are greatly influenced by the length of the incubation period during cultivation. Therefore, this study was conducted to investigate the growth kinetics of five Lipomyces starkeyi strains as determined by biomass production. The five L. starkeyi strains, namely L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8, were inoculated in sterilized Yeast Malt broth, and, incubated for 192 hr at ambient temperature. Biomass yields were assessed and calculated gravimetrically every 24 hr. Results indicated that the optimal biomass production of L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8 were at 120, 168, 144, 168 and 120 hr, with the concentrations of 6.64, 6.43, 9.78, 11.23 and 8.56 g/L, respectively. These results indicate that each L. starkeyi strain requires specific incubation period for the optimum production of fungal biomass. Therefore, by cultivating each L. starkeyi strain at the predetermined incubation period, biomass yields could significantly be improved for further downstream applications such as single cell protein and lipid production.

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Published

2020-12-08

How to Cite

Vincent, M. ., Suali, L. ., Azahari, A. S. ., Mark Baran, P. R. ., Nillian, E. ., & Maurice Bilung, . L. . (2020). Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains. Journal of Advanced Research in Applied Sciences and Engineering Technology, 11(1), 47–54. Retrieved from https://www.akademiabaru.com/submit/index.php/araset/article/view/1959

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