Microwave Irradiation Assisted Synthesis of Silver Nanoparticles using Pullulan as Reducing Agent and Its Antibacterial Activity

Mohd Shahrul Nizam Salleh; Sangetha Cheladorai; Roshafima Rasit Ali; Kamyar Shameli; Zatil Izzah Tarmizi; Justin Chan Zhe

doi:10.37934/jrnn.2.1.4250

Authors

Mohd Shahrul Nizam Salleh Faculty of Chemical Engineering, Universiti Teknologi MARA Cawangan Terengganu, Bukit Besi Campus, 23200 Dungun, Terengganu, Malaysia
Sangetha Cheladorai Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Roshafima Rasit Ali Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Kamyar Shameli Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Zatil Izzah Tarmizi Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
Justin Chan Zhe Department of Chemical Process Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.37934/jrnn.2.1.4250

Keywords:

Silver nanoparticles, microwave, pullulan, antibacterial

Abstract

In this studies, synthesis of silver nanoparticles (Ag-NPs) on pullulan-based biofilm was achieved by microwave irradiation technique. Synthesis of Ag-NPs was achieved using pullulan as both a reducing and stabilizing agent. The effect of different microwave irradiation duration on pullulan and silver nitrate in synthesis of silver nanoparticles (Ag-NPs) was investigated. The synthesized Ag-NPs/PL were first screened and identified using surface plasmon peaks of UV–Vis spectroscopy. The research results indicated that the surface plasmon resonance peaks were observed between 400–414 nm wavelengths in UV-VIS spectroscopy studies. From Fourier-transform infrared spectroscopy (FTIR) spectra, stretching vibrations of hydroxyl (OH), carbonyl (C=O) and C=C stretches exhibits the reduction and stabilization of Ag-NPs. Further, five characteristic peaks Ag(111), Ag(200), Ag(210), Ag(220) and Ag(311) confirmed the presence of elemental silver and the crystalline structure of silver nanoparticles from X-ray Diffraction analysis. Biofilms were produced by mixing the synthesized Pulullan-Ag-NPs with polyvinyl alcohol. The AgNP/PL were applied for the antimicrobial activity against Bacillus subtilis and found to have high antibacterial activity. In addition, the clear zones of inhibition was found at 11 mm to 16 mm against Bacillus Subtillis. The experimental results demonstrated that pullulan could be used as reducing and stabilizing agent for formation of Ag-NPs.

References

S. Coseri, A. Spatareanu, L. Sacarescu, C. Rimbu, D. Suteu, S. Spirk and V. Harabagiu. Green synthesis of the silver nanoparticles mediated by pullulan and 6-carboxypullulan. Carbohydr. Polym., 2015, 13(116), pp. 9–17. doi: 10.1016/j.carbpol.2014.06.008.

Z. Izadiyan, K. Shameli, H. Hara and S. H. M. Taib. Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract. J. Mol. Struct., 2018, 1151(5), pp. 97-105. doi: 10.1016/j.molstruc.2017.09.039.

N. I. S. Abdullah, M. Ahmad, K. Shameli. Biosynthesis of silver nanoparticles using Artocarpus elasticus stem bark extract. Chem. Cent. J., 2015, 9(61), pp. 1-7. doi: 10.1186/s13065-015-0133-0.

P. Suppakul, J. Miltz, K. Sonneveld and S. W. Bigger. Active packaging technologies with an emphasis on antimicrobial packaging and its applications. J. Food Sci., 2003, 68(2), pp. 408-420. doi: 10.1111/j.1365-2621.2003.tb05687.x.

K. Zhang, S. Ai, J. Xie and J. Xu. Comparison of direct synthesis of silver nanoparticles colloid using pullulan under conventional heating and microwave irradiation Inorg. Nano-Metal Chem., 2017, 47(6), pp. 938–945. doi: 10.1080/24701556.2016.1241265.

K. P. Bankura, D. Maity, M. M. R. Mollick, D. Mondal, B. Bhowmick, M. K. Bain, A. Chakraborty, J. Sarkar, K. Acharya and D. Chattopadhyay. Synthesis, characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium. Carbohydr. Polymg., 2012, 89(9), pp. 1159-1165. doi: 10.1016/j.carbpol.2012.03.089.

R. La Spina, D. Mehn, F. Fumagalli, F. Rossi, D. Gilliland, M. Holland and F. Reniero. Synthesis of citrate-stabilized silver nanoparticles modified by thermal and ph preconditioned tannic acid. Nanomaterials., 2020, 10(10), pp. 2031(1–16). doi: 10.3390/nano10102031.

S. W. Jung, Y. Jeong, and S. H. Kim. Characterization of hydrophobized pullulan with various hydrophobicities. Int. J. Pharm., 2003, 254(2), pp. 109-121. doi: 10.1016/S0378-5173(03)00006-1.

K. Shameli, M. Ahmad, E. A. J. Al-Mulla, P. Shabanzadeh and S. Bagheri. Antibacterial effect of silver nanoparticles on talc composites. Res. Chem. Intermed., 2015, 41(1), pp. 251-263. doi: 10.1007/s11164-013-1188-y.

Z. I. Tarmizi, N. I. Arifin, R. R. Ali, K. Shameli, J. C. Zhe and M. S. N. Salleh. Preparation and characterization of polysaccharide mediated copper nanoparticle: The effect of time-varying exposure. IOP Conf. Series: Materials Science and Engineering., 2021, 1051(012096), pp. 1-7. doi: 10.1088/1757-899X/1051/1/012096.

P. Shabanzadeh, N. Senu, K. Shameli, and F. Ismail. Application of artificial neural network for prediction diameter of silver nanoparticles biosynthesized in curcuma longa extract. Digest Dig. J. Nanomater. Biostructures., 2013, 8.(3), pp. 1133-1144.

I. Ristanti, M. Diantoro, H. Wisodo, Hartatiek, A. Taufiq and U. S. Hastuti, Structure, absorbance, and antibacterial activity modification by including Ag-NPs on Fe3O4@polyethylene glycol. AIP Conference Proceedings. 2020, 2234.(1), pp. 040021(1-7). di: 10.1063/5.0008126.

K. X. Lee, K. Shameli, Y. P. Yew, S. Y. Teow, H. Jahangirian, R. Rafiee-Moghaddam and T. J. Webster. Recent developments in the facile bio-synthesis of gold nanoparticles (AuNPs) and their biomedical applications. Int. J. Nanomed., 2020, 15, pp. 275-300. doi: 10.2147/IJN.S233789.

P. Shabanzadeh, N. Senu, K. Shameli, F. Ismail, A. Zamanian, and M. Mohagheghtabar. Prediction of silver nanoparticles’ diameter in montmorillonite/chitosan bionanocomposites by using artificial neural networks. Res. Chem. Intermed., 2015, 41(5), pp. 3275-3287. doi: 10.1007/s11164-013-1431-6.

P. Shabanzadeh, R. Yusof, and K. Shameli. Modeling of biosynthesized silver nanoparticles in Vitex negundo L. extract by artificial neural network. RSC Advances., 2015, 5(106), pp. 87277-87285. doi: 10.1039/C5RA11940E.

P. Shabanzadeh, N. Senu, K. Shameli and M. Mohaghegh Tabar. Artificial intelligence in numerical modeling of silver nanoparticles prepared in montmorillonite interlayer space. J. Chem., 2013, 2013, pp. 1-8. doi: 10.1155/2013/305713.

R. Khandanlou, M. Ahmad, H. R. Fard Masoumi, K. Shameli and M. Basri, Rapid adsorption of copper (II) and lead (II) by rice straw/Fe3O4 nanocomposite: optimization, equilibrium isotherms, and adsorption kinetics study. PloS one., 2015, 10(3), pp. 1-19. doi: 10.1371/journal.pone.0120264.

S. K. Balavandy, K. Shameli, and Z. Z. Abidin, Rapid and green synthesis of silver nanoparticles via sodium alginate media. Int. J. Electrochem. Sci., 2015., 2015, 10.(1), 486-497.

R. Pramila and K. V. Ramesh , Biodegradation of low density polyethylene (LDPE) by fungi isolated from marine water. Afr. J. Microbiol. Res., 2011, 5(28), pp. 5013-5018. doi: 10.5897/AJMR11.670.

S. Khalid, L. Yu, M. Feng, L. Meng, Y. Bai, A. Ali, H. Liu and L. Chen, Development and characterization of biodegradable antimicrobial packaging films based on polycaprolactone, starch and pomegranate rind hybrids. Food Packag. Shelf Life., 18, pp. 71-79. doi: 10.1016/j.fpsl.2018.08.008.

M. Yilmaz, H. Soran and Y. Beyatli, Antimicrobial activities of some Bacillus spp. strains isolated from the soil, Microbiol. Res., 2006, 161(2), 127-131. doi: 10.1016/j.micres.2005.07.001.

M. Balouiri, M. Sadiki and S. K. Ibnsouda, Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 2016, 6(2), pp. 71-79. doi: 10.1016/j.jpha.2015.11.005.